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BOOK TITLE: Principles and Practice of Assisted Reproductive Technology
Troubleshooting” is defined as a form of problem solving, applied to repair failed products or process. The assisted reproductive technology (ART) represents multiple complex systems and the symptoms of a problem can have many possible causes. Troubleshooting involves solving any problem related to clinical or laboratory parameters so as to get the optimum outcome from an in vitro fertilization (IVF) cycle. Right from patient selection, choosing the appropriate stimulation protocol, right choice of gonadotropins, right dose and quality control of drugs, a well-monitored stimulation, appropriate dose and timing of human chorionic gonadotropin (hCG) trigger, and laboratory conditions—they all put together finally affect the success rate of an IVF cycle. In laboratory parameters, success of an ART cycle depends upon a good and well-maintained IVF laboratory as well. By following the basic principles in IVF laboratory we can strive for good outcome. Troubleshooting in cryopreservation requires an understanding of basic principles of cryobiology.