Introduction to Microbiology Department

Apurba Sankar Sastry; Sandhya Bhat K

SECTION 1

1General Microbiology

Section Outline

1. Introduction to Microbiology Department
2. Microscopy
3. Simple Staining
4. Gram Staining
5. Special Staining
6. Bacterial Morphology, Growth
7. Sterilization and Disinfection
8. Culture Media
9. Culture Methods
10. Specimen Collection, Direct Microscopy, Processing for Culture and Motility Testing
11. Conventional Identification of Bacteria (Biochemical Reactions)
12. Automated Culture and Molecular Techniques
13. Antimicrobial Susceptibility Testing2

Introduction to Microbiology DepartmentCHAPTER 1

This is the first practical class in the Department of Microbiology. In this class, a formal interaction between students and teachers (faculty/postgraduate students) takes place where:

The students and the teacher introduce themselves.
Batch distribution: Students are divided into batches and bench teachers are allotted for each batch.
Departmental tour: Students are taken for the departmental tour. Activities/investigations carried out in each section are briefed to the students.
General instructions to be followed during the practical classes are conveyed to the students.

GENERAL INSTRUCTIONS

The following instructions to be followed during the practical classes are conveyed to the students:

Students should wear clean ironed white coat.
Students are warned about the importance of attendance and coming to the class on time.
Practical record: Students must get the practical record in the class every time; which must be filled and signed by the bench teacher on time.
Girls should put their hair inside the coat to avoid infection.
Nails should be kept clean and short. No artificial nails should be worn.
Personal belongings and bags should not be kept near work benches.
No eatables should be brought into the student's laboratory.
Do not put your fingers into your mouth during the practical class hours.
Care of the microscope: Students are instructed how to handle and care the microscope (explained in Chapter 2).
All the slides must be stained on staining rod over the wash basin.
Cap all the reagent bottles and place them in order in the rack after use.
In case of accidental spillage of reagents, inform the bench teacher immediately.
In case of accidental contact of cultures or infectious materials, wash hands with the hand-wash solution immediately and inform the bench teacher.
While leaving the hall: Switch off the microscope, clean the lenses and discard the slide in the discarding jar after use.
Handwashing: Students must perform handwash at the end of every practical class before leaving the hall. Students are demonstrated how to perform handwash. The steps of handwash are demonstrated and explained to them (Fig. 1.1).

Fig. 1.1: Steps of handrub and handwashSource: World Health Organization

Chapter Notes

Introduction to Microbiology DepartmentCHAPTER 1