Clinical Microbiology & Parasitology (For DMLT Students) Nanda Maheshwari
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1Microbiology2

General BiologyCHAPTER 1

Biology is the study of all living organisms (bio = life; logy = study).
 
CLASSIFICATION
The recent system of classification proposed by RH Whittaker in 1969 is a five kingdoms system. Under this system, all living organisms are grouped into five kingdoms as explained below.
 
1. Monera
This group consists of individuals which are single-celled. These may or may not move; they have a cell wall. Nucleus, chloroplasts, other organelles are absent. No visible feeding mechanism. They absorb nutrients through cell wall. Animals with such cells are called prokaryotes.
Examples: Bacteria and blue-green bacteria.
 
2. Protista
Protista are single-celled tiny organisms. These usually move by cilia, flagella, or by amoeboid mechanisms. There is usually no cell wall, although some forms may have a cell wall. They have organelles including a nucleus and may have chloroplasts, so some will be green, while others will not be green in color. Nutrients are acquired by photosynthesis, ingestion of other organisms, or both. Animals with such cells are called eukaryotes.
Examples: Amoeba, Diatom, Euglena, Paramecium and some algae (unicellular), etc.
 
3. Fungi
Fungi are multicellular, with a cell wall, organelles including a nucleus, but no chloroplasts. They have no mechanisms for locomotion. Fungi4 range in size from microscopic to very large (such as mushrooms). Nutrients are acquired by absorption. For the most part, fungi acquire nutrients from dead and decaying material.
Examples: Mushroom, Mucor, Puffball, Yeast, etc.
 
4. Plantae
Plants are multicellular and most do not move, although gametes of some plants move using cilia or flagella. Organelles including nucleus, chloroplasts are present they have a cell wall. Nutrients are acquired by photosynthesis (they all require sunlight).
Examples: Multicellular algae, mosses, ferns, flowering plants (dandelions, roses, etc.), trees, etc.
 
5. Animalia
Animals are multicellular, and move with the aid of cilia, flagella, or muscular organs based on contractile proteins. They have organelles including a nucleus, but no chloroplasts or cell walls. Animals acquire nutrients by ingestion.
Examples: Sponge, jellyfish, insect, fish, frog, bird, man, etc.
All living cells can be categorized into two types as discussed in the following section.
 
PROKARYOTES AND EUKARYOTES
Prokaryotes: (from Old Greek pro: before + karyon: nucleus)
These are organisms without a cell nucleus, or any other membrane-bound organelles. Most are unicellular, but some prokaryotes are multicellular.
Eukaryotes: (from Old Greek eu: good or true + karyon: nucleus)
These are organisms whose cells are organized into complex structures by internal membranes and a cytoskeleton. The most characteristic membrane-bound structure is the nucleus. This feature gives them their name.
Prokaryotes were the only form of life on Earth for millions of years until more complicated eukaryotic cells came into being through the5 process of evolution. Flow chart 1.1 illustrates the type of cells in the organisms of different kingdoms.
 
Comparison Chart
Eukaryotic cell
Prokaryotic cell
Nucleus
Present
Absent
Number of chromosomes
More than one
One, but not true chromosome—Plasmids
Cell Type
Usually multicellular
Usually unicellular (some cyanobacteria may be multicellular)
True membrane-bound nucleus
Present
Absent
Example
Animals and plants
Bacteria and Archaebacteria
Lysosomes and peroxisomes
Present
Absent
Microtubules
Present
Absent or rare
Endoplasmic reticulum
Present
Absent
Mitochondria
Present
Absent
Cytoskeleton
Present
May be absent
Ribosomes
larger
smaller
Golgi apparatus
Present
Absent
Chloroplasts
Present (in plants)
Absent; chlorophyll scattered in the cytoplasm
Permeability of nuclear membrane
Selective
Not present
Plasma membrane with steroid
Yes
Usually no
Cell wall
Only in plant cells and fungi (chemically simpler)
Usually chemically complexed
Cell size
10–100 μm
1–10 μm
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Flow chart 1.1: Types of cells in different kingdoms