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Laboratory Biosecurity: Balancing Risks, Threats & Progress
Ramnik Sood, Najat Rashid
CHAPTER 1:
Introduction
BACKGROUND
RISK ASSESSMENT
RISK MANAGEMENT METHODOLOGY
CHAPTER 2:
Risk Groups
RISK GROUPS CLASSIFICATION
CHAPTER 3:
Biosafety Levels
SAFETY LEVELS
Biosafety Level 1 (Risk Group 1—Minimal Risk)
Biosafety Level 2 (Risk Group 2—Moderate Risk)
Biosafety Level 3 (Risk Group 3—High Risk)
Biosafety Level 4 (Risk Group 4—Very High Risk)
LABORATORY BIOSECURITY AS A COMPLEMENT TO LABORATORY BIOSAFETY
CHAPTER 4:
Laboratory Biosecurity
SAFE AND SECURE LABORATORIES
BIOSECURITY CODES
Codes of Ethics (Aspirational)
Codes of Conduct (Educational/Advisory)
Codes of Practice (Enforceable)
Similarities
Differences
DEVELOPING A BIOSECURITY PROGRAM
CHAPTER 5:
Laboratory Biosecurity: Practical Aspects
LABORATORY BIOSECURITY IN PRACTICE
CHAPTER 6:
Personnel Accountability
EMPLOYEE ACCOUNTABILITY
Personnel Management
Pre-employment Examination
Delegation of Responsibility
Employee Responsibility
Managing Conflicts
CHAPTER 7:
Material Inventory Control
SAFE MATERIAL INVENTORY MANAGEMENT AND CONTROL
ACCESS CONTROL AND MONITORING
DEVELOPMENT OF SOPS
TECHNICAL SOPS
ADMINISTRATIVE OR FUNDAMENTAL PROGRAMMATIC SOPS
CHAPTER 8:
Information Security
DATA SECURITY
CHAPTER 9:
Physical Security
THEFT PROOF LABORATORIES
CHAPTER 10:
Transportation Security
TRANSFER AND TRANSPORT SECURITY
Packing and Transportation of Hazardous Infectious Pathogens
Infectious Substances
Marking and Labeling
The Triple Packing System
Spill Clean-up Procedure
CHAPTER 11:
Biosecurity Emergencies and Apt Responses
BIOSECURITY EMERGENCIES, RESPONSE PLANNING AND NOTIFICATION
CHAPTER 12:
Training and Evaluation
APPROPRIATE LABORATORY PERSONNEL EDUCATION AND TRAINING
PRACTICE DRILLS AND RE-EVALUATIONS
CHAPTER 13:
Technical Aspects of Biosecure Laboratories
TECHNICAL ASPECTS OF LABORATORIES FACILITIES REQUIREMENTS
BSL-4 GENERAL BIOSECURITY REQUIREMENTS
CHAPTER 14:
Laboratory Equipments
BIOLOGICAL SAFETY CABINET
CLASSES AND CHARACTERISTICS OF BSCS
Class I BSC
Class II BSC
Class II, Type A1 Cabinets
Class II, Type A2 Cabinets
Class II, Type B1 Cabinets
Class II, Type B2 Cabinets
Class III Cabinets
FACTORS TO CONSIDER WHILE CHOOSING A BSC
LOCATION OF BSC IN THE LABORATORY
DUCT INSTALLATION FOR EXHAUST AIR
BSC INTERNATIONAL CERTIFICATION AND ACCREDITATION
Certification Tests on the Performance of the BSCs
Primary Test
Secondary Test
GENERAL OPERATING GUIDELINES FOR OPEN-FRONT BSC
USE OF THE BSC
Post-completion Procedures
DECONTAMINATION OF THE BSCS
Gas Decontamination Should be Carried Out
How to Handle Spillage Inside a BSC ?
POLICY ON USE OF UNCERTIFIED BIOSAFETY CABINETS
Beware!
Horizontal Laminar Flow Clean Bench or Work Station
Vertical Laminar Flow Clean Bench or Workstation
AIR FILTRATION SYSTEM
CENTRIFUGE
Types of Laboratory Centrifuges
Catastrophic Failure
General Guidelines for Using the Centrifuge
Preparation
Loading Centrifuge
Operating Centrifuge
Retrieving Samples
Equipment Malfunction
Spill within Equipment
Effective Practices and Techniques in Using Centrifuge
Centrifuge Safety and Biosecurity Aspects
Rotor Safety
Biohazard Safety
User Training
LABORATORY AUTOCLAVE
Safe Usage of Autoclave
General Guidelines for Using Autoclave
Preloading
Loading
Opening
Additional Practices for Autoclaving Liquid
Additional Practices for Autoclaving Dry Loads
PREPARATION OF MATERIALS FOR STERILIZATION
Packaging
Monitoring the Efficiency of Sterilization
Procedure
Prepare a Test Pack for Steam Sterilizer Containing
Autoclave Maintenance
Daily and Weekly Maintenance
Yearly Maintenance
CHAPTER 15:
Laboratory Attire
PERSONAL PROTECTIVE EQUIPMENT AND CLOTHING
Appropriate Laboratory Attire
Personal Protective Equipment
Bibliography
INDEX
TOC
Index
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