Andrology Laboratory Manual Kamini A Rao, Ashok Agarwal, MS Srinivas
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1Andrology Laboratory Manual2
3Andrology Laboratory Manual
Editors Kamini A Rao DGO DORCP DCh FRCOG (UK), MCh (UK), FICOG, PGDMLE (Law) Medical Director, Bangalore Assisted Conception Centre 6/7 Kumara Krupa Road High Grounds Bengaluru, Karnataka, India Ashok Agarwal PhD, HCLD (ABB) Director, Andrology Laboratory and Reproductive Tissue Bank Director, Center for Reproductive Medicine and ART Training Professor, Cleveland Clinic Lerner College of Medicine Case Western Reserve University, Staff, Glickman Urological and Kidney Institute, Ob-Gyn and Wenen's Health Institute, Anatomic Pathology and Immunology, Cleveland Clinic 9500 Euclid Avenue Desk A 19.1 Cleveland, Ohio 44195, USA MS Srinivas PhD Scientific Director and Chief Embryologist Bangalore Assisted Conception Centre, Bengaluru, Karnataka, India
4Published by
Jitendar P Vij
Jaypee Brothers Medical Publishers (P) Ltd
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Andrology Laboratory Manual
© 2010, Jaypee Brothers Medical Publishers (P) Ltd
All rights reserved. No part of this publication should be reproduced, stored in a retrieval system, or transmitted in any form or by any means: electronic, mechanical, photocopying, recording, or otherwise, without the prior written permission of the editors and the publisher.
First Edition: 2010
9788184489019
Typeset at JPBMP typesetting unit
Printed at Ajanta Offset
5CONTRIBUTORS 9FOREWORD
It gives me great pleasure to write the foreword to this book Andrology Laboratory Manual. Its aim is to provide detailed information for the interdisciplinary team of specialists from the clinical members: gynecologists, endocrinologists and urologists to the clinical scientists and embryologists. It encompasses both the latest laboratory techniques and clinical treatments. Our fundamental knowledge is rejuvenated with a concise overview of spermatogenesis, sperm maturation and the genetics of male infertility. Our understanding of the complexity and vulnerability of these processes is enhanced by up-to-date information of the latest advances in male infertility evaluation. Chapters for clinicians include novel information on endocrine disorders, compromising lifestyle factors, sexual dysfunction and surgical sperm retrieval techniques. Chapters for scientists focus on laboratory skills in sperm preparation, protection from infection and cryopreservation. Its format makes it useful as an introductory text and also as an everyday reference resource for those working in the field of Clinical Andrology. I recommend it as a useful, accessible book for every fertility center.
Sheena EM Lewis
Professor of Reproductive Medicine
Centre for Public Health
Queen's University of Belfast
Institute of Clinical Science
Northern Ireland, UK
10
11PREFACE
Today one can find a good number of books written on infertility, but very few which are devoted solely to male infertility and its investigations and management. Most of the books on andrology concentrate on the scientific and some specific aspects of clinical andrology without much attention to male infertility.
Application of Assisted Reproductive Technologies (ART) in dealing with male infertility is in the hands of gynecologists, physicians, urologists and embryologists. This book is an excellent reference for beginners and also for those who are practicing reproductive medicine to update their knowledge in the field of male infertility investigation and management. More importantly, the book elaborates the laboratory aspects in detail, providing reliable information to clinicians to decide the course of therapy to deal with male infertility effectively.
The Andrology Laboratory Manual covers a wide variety of topics including basic sperm biology, male reproductive endocrinology, physiology of sperm maturation and fertilization, genetics issues of male infertility, cryopreservation, surgical sperm retrieval, sexual dysfunction and the effect of environmental factors on semen parameters. Contributions from renowned national and international experts in the field have ensured a standard of excellence comparable to the best in the field.
This volume will serve as a textbook for newcomers to andrology while also providing the experienced physician with a valuable reference manual. It will appeal to all concerned with male reproductive health—andrologists, urologists, embryologists, endocrinologists, gynecologists, as well as basic scientists. The end aim of the book is to help in optimizing results and generate ART treatment of high quality and we hope that it works towards this end.
Kamini A Rao
Ashok Agarwal
MS Srinivas
12
13ACKNOWLEDGMENTS
The editors would like to thank all those involved with the writing and publication of this book. The authors of various chapters who have taken time out from their busy schedules to contribute generously, we thank them for their excellent and well-researched articles. A special thanks goes out to Dr Alex for his excellent contributions as well as his unstinting efforts and involvement in every stage of bringing out this book.