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Practical Manual of Medical Microbiology CP Prince

1:Laboratory Safety
2:First Aid
3:Hand Washing
- INTRODUCTION
  - Method
4:Units
5:Microscope
- INTRODUCTION
  - Types of Microscopes
  - Parts of Compound Microscope
- MECHANICAL PARTS
- MAGNIFYING PARTS
- ILLUMINATING PARTS
- MAGNIFICATION
  - Adjustment of Microscope
  - Care of Microscope
6:Micrometry
- INTRODUCTION
7:Sterilisation
8:Hanging Drop Preparation
- INTRODUCTION
9:Preparation and Fixation of Smears
10:Methylene Blue Staining
- INTRODUCTION
11:Indian Ink Staining
- INTRODUCTION
  - Staining Procedure
  - Observation
12:Gram's Stain
- INTRODUCTION
13:Ziehl-Neelsen's Stain (Acid Fast Stain)
14:Albert's Stain
- INTRODUCTION
15:Leishman's Stain
- INTRODUCTION
16:Preparation and Cleaning of Glassware
- NEW GLASSWARES
17:pH in Microbiology
18:Bacteriological Media
19:Inoculation of Culture Media
20:Anaerobic Cultivation
- SOURCE OF ANAEROBES
  - Methods of Creating Anaerobiosis
  - McIntosh and Filde's Anaerobic Jar
21:Important Bacterial Pathogens and the Diseases
- BACTERIAL PATHOGENS AND RELATED DISEASES
22:Collection of Clinical Materials for Microbiological Investigations
- A. SPECIMEN FOR BACTERIOLOGICAL INVESTIGATIONS
- B. SPECIMENS FOR ACID FAST BACILLI
- C. SPECIMENS FOR ANAEROBIC CULTURE
- D. BLOOD FOR SEROLOGICAL TESTS
- E. MATERIALS FOR MYCOLOGICAL INVESTIGATIONS
- F. SPECIMENS FOR PARASITOLOGICAL EXAMINATION
- G. SPECIMENS FOR VIROLOGICAL INVESTIGATION
- H. MATERIALS FOR STERILITY TESTS
23:Biochemical Tests and Identification of Bacteria
24:O/F Test (Hugh and Leifson's Test)
25:Catalase Test
- PRINCIPLE
26:Oxidase Test
- PRINCIPLE
27:Sugar Fermentation Test
- PRINCIPLE
28:Nitrate Reduction Test
- PRINCIPLE
29:Hydrogen Sulphide Production Test
- PRINCIPLE
30:Urease Test
- PRINCIPLE
31:Citrate Utilisation Test
- PRINCIPLE
32:Voges-Proskauer Test (VP Test)
- PRINCIPLE
33:Methyl Red Test (MR Test)
- PRINCIPLE
34:Indole Test
- PRINCIPLE
35:Bile Solubility Test
- PRINCIPLE
36:Coagulase Test
- PRINCIPLE
37:Antimicrobial Susceptibility Testing
- INTRODUCTION
- MODIFIED KIRBY-BAUER METHOD
38:Brucella Agglutination Test
- PRINCIPLE
39:Anti-Streptolysin O (ASO) Test
- PRINCIPLE
40:CRP Screen Latex Agglutination Slide Test
- INTRODUCTION
  - Principle
  - Requirements
- SPECIMEN
- TEST PROCEDURE
  - Qualitative Estimation
  - Semiquantitative Estimation
- INTERPRETATION OF RESULTS
  - Qualitative Estimation
  - Semiquantitative Estimation
- QUALITY CONTROL
- NOTES
41:VDRL Test
- INTRODUCTION
42:Treponema Pallidum Haemagglutination Assay (TPHA)
- INTRODUCTION
- PRINCIPLE
  - Quantitative Test
  - Observation and Results
43:Widal Test
- INTRODUCTION
- PRINCIPLE
44:Enzyme Linked Immunosorbent Assay (ELISA)
- ELISA FOR ANTIBODY DETECTION
- TYPES OF ELISA
45:Experimental Animals
46:Potassium Hydroxide Wet Mount
47:Lactophenol Cotton Blue Mount
48:Culture of Fungi
- INTRODUCTION
49:Fungal Slide Culture (Riddle's Method)
- INTRODUCTION
50:Identification of Fungal Isolates
- INTRODUCTION
51:Germ Tube Test
- INTRODUCTION
52:Diagnosis of Virus Infections
53:Lab Diagnosis of Malaria
54:Parasitological Examination of Faeces
55:Medical Entomology
- INTRODUCTION
- HOUSE FLY (MUSCA DOMESTICA) (FIG. 55.8)
  - Diseases transmitted (Mechanical vector)
- RAT FLEA (XENOPSYLLA) (FIG. 55.9)
  - Diseases Transmitted
- LOUSE (PEDICULUS HUMANUS) (FIGS 55.10 AND 55.11)
- ITCH MITE (SARCOPTES) (FIG. 55.12)
- TICKS
  - Hard Ticks
- CYCLOPS (WATER FLEAS) (FIG. 55.13)
  - Control of Guinea worm infection by ingestion of cyclops
56:Bacteriological Examination of Water
57:Mocrobiology of Milk
- INTRODUCTION
58:Lab Diagnosis of Tuberculosis
59:Urinary Tract Infection
60:Spotters
- PLATINUM WIRE LOOP (FIG. 19.4)
- CANDLE JAR (FIG. 20.2)
- TESTING OF AUTOCLAVES
- SEITZ FILTER
- CENTRIFUGE (FIG. 22.2)
- THROAT SWAB (FIG. 22.3)
- GLUCOSE BROTH
- GELATIN
- NUTRIENT AGAR
- BLOOD AGAR
- AGAR AGAR
- SABOURAUD'S GLUCOSE AGAR
- STAPHYLOCOCCI(FIG. 60.1)
- HAEMOLYSIS ON BLOOD AGAR
- NEISSERIA GONORRHOEAE
- GRAM POSITIVE BACILLI
- THIOGLYCOLLATE MEDIUM
- ROBERTSON'S COOKED MEAT MEDIUM
- McINTOSH AND FILDE'S JAR
- MAcCONKEY'S AGAR WITH LACTOSE AND NON-LACTOSE FERMENTERS
- MAcCONKEY'S AGAR (FIG. 60.4)
- CLOSTRIDIUM TETANI WITH TERMINAL SPORES
- SUGAR MEDIA TO TEST FOR CARBOHYDRATE FERMENTATION
- GRAM NEGATIVE BACILLI
- RABBIT
- MICE
- GUINEA PIG
- VDRL (VENEREAL DISEASE RESEARCH LABORATORY) TEST SLIDE
- WIDAL TEST
- ANTIBIOTIC SENSITIVITY PLATE
- CORYNEBACTERIUM DIPHTHERIAE
- UREASE POSITIVE AND UREASE NEGATIVE REACTIONS
- DERMATOPHYTE ON SABOURAOUD'S AGAR
- CANDIDA IN GRAM'S SMEAR
- ASPERGILLUS
- ASPERGILLUS ON SABOURAUD'S AGAR
- MYCOBACTERIUM TUBERCULOSIS ON LOWENSTEIN-JENSEN MEDIUM
- MYCOBACTERIUM TUBERCULOSIS IN ACID-FAST SMEAR (FIG. 13.1)
- LOWENSTEIN-JENSEN MEDIUM
- OVUM ASCARIS LUMBRICOIDES (ROUNDWORM) (FIG.54.9)
- OVUM OF ANCYLOSTOMA DUODENALE (HOOKWORM) (FIG. 54.6)
- OVUM OFTRICHURIS TRICHURA(WHIP WORM) (FIG. 54.7)
- MALARIAL PARASITE (GAMETOCYTE)
- MICROFILARIA
- MALARIAL PARASITE (TROPHOZOITE)
- HOOKWORM (ANCYLOSTOMA DUODENALE) (FIG. 54.6)
- ROUND WORM (ASCARIS LUMBRICOIDES) (FIG. 54.9)
- WHIP WORM (TRICHURIS TRICHURA) (FIG. 54.7)
- HYDATID CYST (FIG. 60.5)
- TAPE WORM (FIG. 60.6)

INDEX

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Index

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Chapter Notes