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Chapter-19 Estimation of serum albumin by Dye-binding method

BOOK TITLE: Laboratory Manual for Practical Biochemistry

Author

1. MK Ganesh
2. A Hemavathi
3. YM Shivaraja Shankara

ISBN

9788184482591

DOI

10.5005/jp/books/10435_20

Edition

1/e

Publishing Year

2008

Pages

Author Affiliations

1. KVG Dental College, Sullia, Karnataka, KVG Dental College, Sullia, Karnataka, India
2. KVG Medical College, Sullia, Karnataka
3. KVG Medical College, Sullia, Karnataka, KVG Medical College, Sullia, Karnataka, India

Chapter keywords

Abstract

Abstract

Many proteins have properties to bind certain dyes. Especially albumin binds specifically to several anionic dyes such as bromocresol green (BCG). The albumin – BCG complex absorbs maximally at 630 nm. The intensity of the color formed is directly proportional to albumin concentration in the serum. The interference from other serum proteins are very much minimal because albumin binds much faster with BCG.

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