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Chapter-11 Flow Cytometry in Acute Leukemia: Practical Aspects

BOOK TITLE: Laboratory Techniques in Haematology

Author
1. A Kumar
2. S Gujral
3. Y Badrinath
ISBN
9788184482201
DOI
10.5005/jp/books/10438_11
Edition
1/e
Publishing Year
2008
Pages
20
Author Affiliations
1. Nalanada Medical College, Patna, Bihar, Tata Memorial Hospital, Mumbai
2. Tata Memorial Hospital, Mumbai
3. Tata Memorial Hospital, Mumbai
Chapter keywords

Abstract

Flow cytometry has emerged as an important technology for immunophenotyping of acute leukemias. This chapter describes in detail, the protocol for sample preparation for flow cytometry, application of flow cytometry in diagnosis of AML, ALL (B and T) and biphenotypic leukemia. It touches upon the role of flow cytometry in minimal residual disease detection, body fluid analysis, FNAC and discusses the differences between flow cytometry and immunohistochemistry. It also elaborates upon the trouble shooting in flow cytometry.

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