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Chapter-14 Demonstration of Unstable Haemoglobin

BOOK TITLE: Laboratory Procedures in Haematology

Author

1. Mehdi SR

ISBN

9788180616570

DOI

10.5005/jp/books/10437_14

Edition

1/e

Publishing Year

2006

Pages

Author Affiliations

1. JN Medical College, AMU, Aligarh, India, Era’s Lucknow Medical College, Lucknow, Uttar Pradesh, India

Chapter keywords

Abstract

Abstract

Normal haemoglobin is stable when heated in a waterbath at 50°C while the unstable haemoglobins like haemoglobin E are not and they are precipitated. The haemolysate for the test sample is mixed with the buffer of pH 7.4 and heated at 50°C if unstable haemoglobin is present a copious flocculent precipitate is formed within 60 minutes. The test is sensitive and specific for unstable haemoglobin.

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