Introduction: Maintenance of good oral hygiene helps in preventing dental diseases. One of the primary etiologies for the development of dental diseases is plaque. The development of a complex flora known as mature dental plaque on the surface of the tooth begins with a small number of pioneer microbial species. Microorganisms like Streptococcus mutans and Candida albicans are predominantly found in dental plaque and are associated with caries lesions. It is an established fact that S. mutans plays a vital role in initiating dental caries and C. albicans has been shown to enhance the adherence of S. mutans to the tooth surface. Dentifrices play a pivotal role in the removal of dental plaque. The market is flooded with various types of herbal and nonherbal toothpaste. Aim and objective: To evaluate the antimicrobial efficacy of commercially available toothpastes on C. albicans and S. mutans isolated from the saliva of patients with early childhood caries (ECC). Materials and methods: This ex vivo study was conducted on 40 children with ECC (aged 3–6 years). Saliva samples were collected and sent for microbial analysis. Positive samples of C. albicans and S. mutans were divided into two groups of 20 each. Five toothpastes and distilled water as the control group were used to test the microorganisms. The agar well diffusion method was used to check the effectiveness of the toothpastes. Results: Dabur Meswak showed the maximum zone of inhibition against S. mutans and C. albicans. Conclusion: The regular use of herbal toothpaste is an alternative to traditional toothpaste because of its effectiveness and chemical-free nature.