Platelet-rich plasma (PRP), prepared from autologous blood, is commonly used in various pathologies like hip and knee osteoarthritis, rotator cuff pathology, epicondylitis, tendinitis, fracture healing and back pain, for tissue regeneration, wound healing, scar revision, skin rejuvenating effects, and also in treatment of alopecia. It is one of the important products, which is nowadays being used by pain physicians aggressively. But, the preparation protocols vary and there are many factors that influence the composition of PRP. The aim of this review is to find the details of PRP and the standard preparation protocol, if any.