Changing patterns of antibiotic sensitivity of V. cholerae is a major concern as Multiple-Antibiotic-Resistant (MAR) V. cholerae isolates are being reported from various parts of the world. The study was conducted to analyze the prevalence and antibiotic resistance pattern of V. cholerae O1. Samples were processed according to the standard guidelines. Sporadic outbreaks occurred in June 2004 and March 2010. Antibiotic resistance was seen in the strains isolated. Alarge number of cholera epidemics have been associated with MAR strains of V. cholerae. This makes it imperative that all isolates be constantly subjected to susceptibility testing and resistance patterns to each antibiotic be monitored.