Aim: To assess the role of porous collagen in deep sclerectomy (DS), with and without trabeculo-Descemet membrane (TDM) rupture. Patients and methods: Forty-six eyes with different types of open-angle glaucoma and medically uncontrolled intraocular pressure (IOP) were selected. DS was performed in all cases. Ologen was implanted as a single large piece in the scleral lake and subconjunctival space in all cases with and without TDM rupture. Results: A total sample of 46 open-angle glaucoma patients were included in the study. The mean ± standard deviation (SD) IOP was 25.6 mm Hg ± 10.6 (range 12–58 mm Hg) preoperatively. On follow-up, the mean ± SD IOP was 6.1 mm Hg ± 3.7 (range 2–20 mm Hg) 1 day postoperatively and 9.3 mm Hg ± 4.0 (range 4–23 mm Hg) after 1 month of follow-up, at 12 months the IOP was at 12.1 mm Hg ± 3 (range 8–18 mm Hg). The overall mean ± SD IOP reduction percentage was 48.3% ± 21.3 (range 0.0–86.2). Comparing mean IOP reductions at last follow-up between TDM rupture cases and non-TDM cases (Mann–Whitney test), the mean ± SD IOP reduction in TDM rupture patients was 12.1 ± 8.0 mm Hg (range 2-27) meanwhile, in non-TDM rupture patients it was 14.3 ± 11.4 mm Hg (range 0–50). However, the difference in IOP reduction between the two groups was not significant. (p = 0.689) Conclusion: Porous collagen can enhance the results of DS; also, it helps to proceed with DS in cases of TDM rupture without converting to trabeculectomy.