Introduction: Surgical site infections (SSIs) after major abdominal surgeries are the major causes of morbidity and mortality in our country. SSIs can be prevented by usage of antimicrobial prophylaxis. The appropriate administration of antimicrobial prophylaxis, with respect to choice and timing, is crucial to yield better results and reduce the prevalence of SSIs. Aims: To analyze the time, duration, route, and choice of antimicrobial prophylaxis in major abdominal surgeries at a tertiary care hospital and to assess the prevalence of surgical site wound infections. Methodology: We conducted a descriptive, observational, and cross-sectional study on 100 patients who were administered antimicrobial prophylaxis preoperatively before undergoing major abdominal surgeries. The time, duration, route, and choice of antimicrobial prophylaxis for 18 different types of abdominal surgeries, which included gastroduodenal and gynecological cases, were recorded. These patients were followed up postoperatively for 30 days following the surgery. Incidence of SSIs was also recorded. Results: The prevalence of SSIs in major abdominal surgeries in this study was found to be 7%, which is on the lower limit when compared to similar studies involving major abdominal surgeries conducted in India. This is attributed to proper administration of antimicrobial prophylaxis, with respect to their choice, dosage, and time of administration. The prevalence of SSIs was predominantly seen in elderly groups of patients of advancing age, patients with higher American Society of Anesthesiology (ASA) score, patients undergoing open surgeries, and in prolonged surgical procedures that exceeded their usual duration. Conclusion: This study of antimicrobial prophylaxis for major abdominal surgeries conducted at tertiary care hospitals has shown the prevalence of SSIs as 7%. The prevalence of SSIs is low due to the appropriate choice and timing of administration of antimicrobial prophylaxis.