Effect of Chronic Stress on Ligature-induced Periodontitis in Inbred and Noninbred Rats: A Radiographic Study

JOURNAL TITLE: The Journal of Contemporary Dental Practice

Author
1. Fábio Luis Miranda Pedro
2. Alex Semenoff-Segundo
3. Mateus Rodrigues Tonetto
4. Matheus Coelho Bandeca
5. Alessandra Nogueira Porto
6. Reidson Stanley Soares dos Santos
7. Tereza Aparecida Delle Vedove Semenoff
8. Alvaro Henrique Borges
ISSN
DOI
10.5005/jp-journals-10024-1578
Volume
15
Issue
5
Publishing Year
2014
Pages
5
Author Affiliations
    1. Department of Restorative Dentistry, UNESP Araraquara (FOAr), São Paulo, Brazil
    1. University of Cuiaba, Cuiaba, Brazil
    1. University of Cuiaba - UNIC, Cuiaba, Brazil
    1. Post-Graduation in Dentistry, CEUMA University São Luis, MA, Brazil
    1. Department of Master Program in Integrated Dentistry Science, University of Cuiabá (UNIC), Cuiabá, Mato Grosso Brazil
    1. Department of Master Program in Dentistry, CEUMA University - UniCEUMA, São Luis, Maranhao, Brazil
    1. Department of Master Program in Integrated Dentistry Science, University of Cuiabá (UNIC), Cuiabá, Mato Grosso Brazil
    1. Department of Master Program in Integrated Dentistry Science, University of Cuiabá (UNIC), Cuiabá, Mato Grosso Brazil
  • Article keywords

    Abstract

    Aim

    The aim was to compare the effect of chronic stress on ligature-induced periodontitis in inbred and noninbred female rats by means of a radiographic study.

    Materials and methods

    Adult Lewis (inbred) and Wistar (noninbred) rats were used and divided into the following groups: Ligature/Stress Lewis Group (LSLG, n = 8); Ligature/Stress Wistar Group (LSWG, n = 8); Ligature Lewis Group (LLG, n = 8) and Ligature Wistar Group (LWG, n = 8). The animals were anesthetized and a silk thread was continuously kept around their second upper right molar. Then, groups LSLG and LSWG were subjected to physical stress test (physical restraint for 12 hours). After 15 days of ligature placement, the animals were euthanized. The right hemimaxilla was kept in formalin solution for 48 hours. Radiographs of the hemimaxillae were obtained using the parallelism technique and subsequently submitted to a standardized radiographic processing. The examiner was blinded to the groups and calibrated. The bone height level was measured and the data were subjected to statistical analysis (ANOVA, Tukey, p < 0.05).

    Results

    LSWG showed bone destruction significantly higher than that of LSLG (32.1 ± 1.45 mm and 23.6 mm ± 2.13, respectively). Similarly, LWG showed bone destruction significantly higher than that of LLG (28.6 ± 1.18 mm and 25.2 ± 2.76 mm, respectively).

    Conclusion

    Inbred rats (Lewis) are less susceptible to the effects of chronic stress than are noninbred rats (Wistar) in relation to ligature-induced periodontitis.

    Clinical significance

    Epidemiological studies have shown a relationship between stress and periodontitis. One of the major difficulties of this type of research is the bias of the exact diagnosis of the patient's emotional state. Inbred lines animals have standards-specific neuroendocrine, this allows answers about the susceptibility of periodontal disease, making knowledge applicable in future clinical trials.

    How to cite this article

    Semenoff-Segundo A, Borges AH, Bandeca MC, Porto AN, Pedro FLM, dos Santos RSS, Tonetto MR, Semenoff TADV. Effect of Chronic Stress on Ligatureinduced Periodontitis in Inbred and Noninbred Rats: A Radiographic Study. J Contemp Dent Pract 2014;15(5):556-560.

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