Staining the Ground Section of the Tooth Using an Innovative Plant Stain Found in the Pravara Region, India

JOURNAL TITLE: The Journal of Contemporary Dental Practice

Author
1. Prasad N Mhaske
2. Chaitra S Mastud
3. Santoshkumar P Mastud
4. Tejashree Mantri
5. Deepak M Vikhe
ISSN
DOI
10.5005/jp-journals-10024-2904
Volume
21
Issue
10
Publishing Year
2020
Pages
4
Author Affiliations
    1. Department of Prosthodontics, Rural Dental College and Hospital, Pravara Institute of Medical Science (DU), Pravara Medical Trust, Loni, Maharashtra, India
    1. Department of Orthodontics, Dr DY Patil Vidyapeeth, Dr DY Patil Dental College and Hospital, Pimpri, Pune, Maharashtra, India
    1. Department of Oral Medicine and Radiology, Maharashtra Institute of Dental Sciences & Research, Latur, Maharashtra India
    1. Department of Prosthodontics, Bharati Vidyapeeth University Dental College, CBD Belapur, Navi Mumbai, Maharashtra, India
    1. Department of Oral Medicine and Radiology, Dr DY Patil Vidyapeeth. Dr DY Patil Dental College and Hospital, Pimpri, Pune, Maharashtra, India
  • Article keywords

    Abstract

    Aims: To assess the staining efficacy of the innovative “Pravara” stain on ground sections of human teeth under a stereomicroscope. In this study, the ground sections were stained with the innovative stain. The stain is obtained from a leafless tree called Terminalia chebula. Terminalia chebula, commonly known as Harad, is found in South Asia from India. Few species are found in the Pravara region of Maharashtra, India. Materials and methods: A total of 40 extracted teeth were collected and ground sections of each tooth were prepared. The samples were randomly divided into two groups: group I—control (without stain), group II—study group (the samples in this group were stained with the help of innovative “Pravara” stain). The sections were graded from I to III according to the differentiation and contrast of tissues. Results: The ground sections in group II stained with Pravara stain demonstrated better contrast for structures within sections under a microscope in comparison with group I (control group). Conclusion: The study demonstrates the efficacy of a new innovative Pravara stain, which is a simple, economical, quick, and effective staining method for the ground sections of the teeth. Clinical significance: This innovative Pravara stain is cost-effective, saves time, ecofriendly, and highlights the anatomical structures more effectively and does not fade easily with time.

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