Introduction: Pedodontists have always looked for newer techniques, safer materials, and ergonomic technologies to treat pulpal infections so as to help children heal faster. Low-level laser is one such innovation that needs to be assessed for its efficacy as a potential pulpotomy medicament. Aim: To evaluate the histological changes in the dental pulp of dog's primary premolars after pulpotomy using low-level diode laser therapy (LLLT) in comparison with mineral trioxide aggregate (MTA), and Pulpotec. Design: In this experimental study, 30 teeth were randomly selected from five dogs. The animals were anesthetized and pulpotomized using LLLT, MTA, and Pulpotec respectively. One week later, the pulpotomized teeth were extracted and subjected to histological evaluation for inflammation. Histological changes seen in the pulp tissues of all the groups were subjected to statistical analysis using Kruskal–Wallis test for groupwise analysis (p-value of < 0.001) and for pairwise Mann–Whitney U test (p-value of < 0.05). Results: On histologic observations, least inflammation was seen in low-level laser group followed by Pulpotec. Severe inflammatory cell infiltrates were seen in MTA. Conclusion: Low-level laser therapy appears to be a safe, minimally invasive, yet an effective modality for pulpotomy followed by Pulpotec.