Impact of Prematuration Culture on Zygote Morphology in In Vitro Maturation: An Early Clue to Embryo Competence

JOURNAL TITLE: International Journal of Infertility & Fetal Medicine

Author
1. Sumi Maria
2. Mohammed A Cheruveetil
3. Muhammed Asif
4. Prasanna K Shetty
5. Mir Jaffar
6. Arya Rajendran
ISSN
2229-3817
DOI
10.5005/jp-journals-10016-1251
Volume
13
Issue
1
Publishing Year
2022
Pages
10
Author Affiliations
    1. Department of Reproductive Medicine, Milann Fertility Centre, Bengaluru, Karnataka, India
    1. Department of Obstetrics and Gynaecology, KS Hegde Medical Academy, Mangaluru, Karnataka, India
    1. Milann—The Fertility Center (A Unit of BACC Healthcare Pvt Ltd), Bengaluru, Karnataka, India
    2. Milann—The Fertility Center, Bengaluru, Karnataka, India
    3. Valley Fertility Center, Srinagar, Jammu and Kashmir, India
    1. Government Medical College, Amritsar, Punjab, India
    2. Milann—The Fertility Center, Bengaluru, Karnataka, India
    3. NIMS Fertility Centre, NIMS Welcare Hospital, Palakkad, Kerala, India
    4. NIMS Fertility Center, Palakkad, Kerala, India
    5. Nova IVF Fertility, Palakkad, Kerala, India
    1. Department of Embryology, Garbhagudi IVF Center, Bengaluru, Karnataka, India
    1. Milann—The Fertility Center, Bengaluru, Karnataka, India
  • Article keywords
    Cilostamide, Nucleolar precursor bodies, Polar body, Prematuration culture, Pronuclear zygote, Pronuclei

    Abstract

    Background: The study was undertaken to gain insight into the morphology of pronuclear oocytes developed through prematuration culture with cilostamide. The criteria such as position and orientation of pronuclei (PN), the size and distribution of nucleolar precursor bodies (NPB), alignment of polar body (PB), and the cytoplasmic halo have been included in the study. Objective: To elucidate the effect of cilostamide on zygote morphology in prematuration culture. Study design: A prospective analysis of pronuclear zygote morphology developed through IVM with prematuration culture using cilostamide conducted from April 2018 to March 2020. Materials and methods: The present study comprised of 57 zygotes, obtained from the in vitro matured oocytes of 63 patients aged between 25 and 35 years, who underwent controlled ovarian stimulation for IVF/ICSI. Results: In pronuclear morphology, the central juxtaposed position was higher in the experimental group (77.1%) than in the control group (54.5%). The Group 1 NPB distribution was statistically significant in the experimental group (51.4%) as compared to the control group (22.7%) (p = 0.03). Besides, Group 3 NPBs were lower in the experimental group (11.4%) when compared to the control group (36.4%) (p = 0.02). The α-type polar body distribution was significantly higher in the experimental group (65.7%) (p = 0.03). Cytoplasmic halo was noted in the experimental (60%) and control (45.5%) groups (p > 0.05). Conclusion: The results indicate that prematuration culture using cilostamide for synchronizing nuclear and cytoplasmic maturation yielded a better pronuclear zygote morphology.

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