Aim: The aim of this in vitro study was to evaluate and compare the adhesive bond strength of conventional glass ionomer cement (GIC) and Cention N to the primary enamel and dentin using an accelerated fatigue test. Materials and methods: A total of 30 sound human primary molars were collected and were mounted on a metal cylindrical block using acrylic resin, embedding the root up to cemento-enamel junction (CEJ). Proximal box was prepared on both mesial and distal surfaces, one of the cavity was restored with GIC (Type 9) and the other proximal cavity with Cention N. A nonretentive cavity design was followed for both the materials so as to maintain the uniformity between the two specimens were then placed under a universal testing machine (Instron) and subjected to accelerated cyclic loads till a separation fracture occurs at the tooth-restoration interface. The number of endured cycles a particular restoration could withstand before getting fractured was registered. Results: Cention N resisted significantly more number of endured cycles before separation from the cavity as compared to GIC (p < 0.001). Conclusion: Within the limitations of the study, it can be concluded that newly developed material Cention N is preferred alternative over conventional GIC for the restoration of proximal cavities in primary molars.