Comparison of Hybrid Capture 2 Assay with Real-time-PCR for Detection and Quantitation of Hepatitis B Virus DNA

JOURNAL TITLE: Euroasian journal of hepato-gastroenterology

Author
1. Farjana Majid
2. Munira Jahan
3. Ahmed Lutful Moben
4. Shahina Tabassum
ISSN
2231-5047
DOI
10.5005/jp-journals-10018-1093
Volume
4
Issue
1
Publishing Year
0
Pages
5
Author Affiliations
    1. Department of Microbiology, Tairunnessa Memorial Medical College, Tongi, Gazipur, Bangladesh
    1. Department of Virology, Bangabandhu Sheikh Mujib Medical University, Dhaka, Bangladesh
    1. Department of Medicine, Shaheed Suhrawardy Medical College Hospital, Dhaka, Bangladesh
    1. Department of Virology, Bangabandhu Sheikh Mujib Medical University, Dhaka, Bangladesh
  • Article keywords

    Abstract

    Background: Both real-time-polymerase chain reaction (PCR) and hybrid capture 2 (HC2) assay can detect and quantify hepatitis B virus (HBV) DNA. However, real-time-PCR can detect a wide range of HBV DNA, while HC2 assay could not detect lower levels of viremia. The present study was designed to detect and quantify HBV DNA by real-time-PCR and HC2 assay and compare the quantitative data of these two assays. Materials and methods: A cross-sectional study was conducted in between July 2010 and June 2011. A total of 66 serologically diagnosed chronic hepatitis B (CHB) patients were selected for the study. Real-time-PCR and HC2 assay was done to detect HBV DNA. Data were analyzed by statistical Package for the social sciences (SPSS). Results: Among 66 serologically diagnosed chronic hepatitis B patients 40 (60.61%) patients had detectable and 26 (39.39%) had undetectable HBV DNA by HC2 assay. Concordant results were obtained for 40 (60.61%) out of these 66 patients by real-time-PCR and HC2 assay with mean viral load of 7.06 ± 1.13 log10 copies/ml and 6.95 ± 1.08 log10 copies/ml, respectively. In the remaining 26 patients, HBV DNA was detectable by real-time-PCR in 20 patients (mean HBV DNA level was 3.67 ± 0.72 log10 copies/ml. However, HBV DNA could not be detectable in six cases by the both assays. The study showed strong correlation (r = 0.915) between real-time-PCR and HC2 assay for the detection and quantification of HBV DNA. Conclusion: HC2 assay may be used as an alternative to real-time-PCR for CHB patients.

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