We present a case of ischiopagus conjoined twins with a dividing membrane and two yolk sacs at 9 weeks and 1 day of gestation. Two-dimensional sonography clearly showed ischiopagus conjoined twins with a dividing membrane. Color Doppler revealed vascular communication between the conjoined twins in the pubic area and one umbilical cord. HDlive Silhouette clearly depicted ischiopagus conjoined twins with two yolk sacs. To the best of our knowledge, this is the first reported case of ischiopagus conjoined twins with a dividing membrane and two yolk sacs described early in the first trimester of pregnancy.