Manual of Histological Techniques

by Santosh Kumar Mondal

The objective of this manual is to introduce the histological techniques. The relentless progresses of medical technology have brought into its wake novel methods of investigations and treatments, rendering many older methods obsolete. Pathological investigations remain the cornerstone of medical diagnosis. Histotechnology plays a pivotal role to make a diagnosis of surgical specimens. With the advent of newer diagnostic techniques like molecular diagnostic methods, a quick but very reliable diagnosis is possible, even from small amount of tissue. This manual consists of 29 chapters, which include tissue fixation and fixatives, tissue processing, microtomy and paraffin sections, cryostat and frozen section, staining of tissues—basic concept, routine hematoxylin and eosin stain, carbohydrates, lipids, proteins and nucleic acid staining, connective tissue staining, pigments and minerals, bone and decalcification, nervous system, cell block and diagnostic cytopathology, electron microscopy, bone marrow trephine biopsy, tru-cut biopsy or core needle biopsy, microarrays and DNA sequencing etc. For students’ easy understanding; many figures, charts, diagrams and tables have been included. Specialists and consultants who are working in a surgical pathology laboratory will also find it useful.

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