This chapter deals with the discussion on newer diagnostic modalities in assisted reproductive technique (ART). Doppler studies allow evaluation of direction and magnitude of blood flow. 3D ultrasonography (USG) can provide multiplanar images, volume rendering, and surface rendering. Implantation of embryo requires a complex cross-talk between embryo and endometrium which involves over 236 genes expression. The transcriptomic signature of the window of receptivity or window of implantation (WOI) of the endometrium can be identified by advances in microarray technology. Sperm DNA fragmentation index (DFI) denotes the degree of damage to the genetic integrity of sperm DNA. The follicle-stimulating hormone (FSH) and the LH are glycoprotein hormones secreted from anterior pituitary. They are heterodimers with alpha subunit being common to FSH, LH, human chorionic gonadotropin (hCG), and thyroid-stimulating hormone. Recent trends in medical management in ART are corifollitropin alfa/delta/epsilon, synthetic kisspeptins, elagolix, uterine transplantation, bioengineered uterus, ectogenesis, INVO procedure, in vitro gametogenesis, gene editing, and human chorionic gonadotropin injections which are discussed in detail. Advances in embryology laboratory techniques are also covered thoroughly in this chapter.