This chapter presents a review on assisted reproductive technology (ART) guidelines. About 8% of infertile couples, however, need serious medical intervention involving the use of advanced assisted reproductive technology (ART) procedures, such as in vitro fertilization (IVF) or intracytoplasmic sperm injection (ICSI). Such advanced treatment is expensive and not easily affordable to the majority of Indians. Further, the successful practice of ART requires considerable technical expertise and expensive infrastructure. Minimal physical requirements for art clinic include semen collection room, semen processing laboratory, sterile area, Operation Theater, embryology laboratory complex, maintenance of the laboratories, and power supply backup. The practice of ART requires well-organized teamwork between the gynecologist, the andrologist and the clinical embryologist supported by a counselor and a program coordinator or director. The indications for artificial insemination with donor semen (AID) are when there is (a) nonobstructive azoospermia; (b) the husband has a hereditary genetic defect; or (c) when the couples have Rh incompatibility. Oocyte donation or embryo donation, traditional surrogacy or gestational surrogacy, close relative as a surrogate, infertility, assisted reproductive technology bank, etc. are some important topics which are covered under this chapter.