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Ultrasound is an excellent tool for assessment of the menstrual cycle. Hormonal changes occurring day-to-day during the menstrual cycle reflects as morphological and vascular changes in the ovary and the uterus. Ultrasound with Doppler can thus be used as the only modality for cycle assessment in patients undergoing assisted reproduction technology and may be of help to reduce the cost of the cycle by avoiding certain hormonal assessments and still maintaining close and accurate watch on the hormonal changes occurring during treatment cycle. This chapter covers the imaging modalities for assessment of reproductive system, preovulatory period, estrogen affects endometrium, secretory phase assessment, and correlation of progesterone to ultrasound findings.