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Chapter-123 Cryopreservation

BOOK TITLE: Principles and Practice of Assisted Reproductive Technology

Author
1. PS Divyashree
2. VV Bezar Ghan
3. Kumari K Anitha S
4. C Mohammed Ashraf
ISBN
9789352705030
DOI
10.5005/jp/books/18020_124
Edition
2/e
Publishing Year
2019
Pages
22
Author Affiliations
1. Milann—The Fertility Center (A Unit of BACC Healthcare Pvt Ltd), Bengaluru, Karnataka, India, Milann - The Fertility Center, Bengaluru, Karnataka, India, Milann—The Fertility Center, Bengaluru, Karnataka, India, Milann JP Nagar, Bengaluru, Karnataka, India, Genea Fertility Center, Bengaluru, Karnataka, India, Genia Fertility Center, Bengaluru, Karnataka, India
2. Milann—The Fertility Center (A Unit of BACC Healthcare Pvt Ltd), Mangalore, Karnataka, India, Ovum Fertility and Women's Health Center, Bengaluru, Karnataka, India, Craft Fertility Center, Gunasheela IVF Center, Hamad Medical Corporation KMC, YMC, Milann, Qatar, India, Sweden
3. Milann—The Fertility Center (A Unit of BACC Healthcare Pvt Ltd, Bengaluru, Karnataka, India, Milann—The Fertility Center, Bengaluru, Karnataka, India
4. Milann—The Fertility Center, Bengaluru, Karnataka, India, Milann Fertility Centre, Bengaluru, Karnataka, India, Milann Fertility Center, Bengaluru, Karnataka, India
Chapter keywords
Cryopreservation, oocyte, human tubal fluid, equilibration solution, vitrification solution, embryo, warming solution, cryolock, blastocyst cryopreservation, blastocyst warming

Abstract

This chapter summarizes the basic steps of vitrification along with the morphological changes during vitrification and warming. The main principle of cryopreservation for mammalian oocytes and embryos is to reduce damage caused by intracellular ice formation. Vitrification of oocytes contains two solutions, i.e. equilibration solution (ES) and vitrification solution (VS). ES contains no cryoprotectant. The embryos are kept in this solution for a minimum of 5 minutes to a maximum of 10 minutes. Vitrification solution 1 (VS1) contains ethylene glycol as a cryoprotectant. The embryos are transferred from ES to VS1.

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