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Chapter-122 Advanced Techniques

BOOK TITLE: Principles and Practice of Assisted Reproductive Technology

Author
1. Rao Kamini A
2. PS Divyashree
3. Kottur Anu
4. VV Bezar Ghan
5. Kumari K Anitha S
6. C Mohammed Ashraf
7. Kumar R Dileep
ISBN
9789352705030
DOI
10.5005/jp/books/18020_123
Edition
2/e
Publishing Year
2019
Pages
18
Author Affiliations
1. Bangalore Assisted Conception Centre, Bangalore, Milann Fertility Centre, Bangaluru, Karnataka, India, Milann—The Fertility Center, Bengaluru, Karnataka, India, Milann (A Unit of BACC Health care Private Limited), Bengaluru, Karnataka, India, Milann (A Unit of BACC Healthcare Pvt Ltd), Bengaluru, Karnataka, India, Milann (A Unit of BACC, Healthcare Pvt Ltd), Bengaluru, Karnataka, India, Milann Group of Fertility Centers, Bengaluru, Karnataka, India, Bengaluru, Karnataka, India, Bengaluru, Milann—The Fertility Center (A Unit of BACC Healthcare Pvt Ltd), Bengaluru, Karnataka, India, Milann—The Fertility Center (A Unit of BACC Health Care Pvt Ltd), Bengaluru, Karnataka, India, Bangalore Assisted Conception Centre, Bangalore, Karnataka, India, BACC Health Care Private Limited, Bengaluru, Karnataka, India, Bangalore Assisted Conception Center (BACC), Bengaluru, Karnataka, India, FOGSI-2001; Bangalore Assisted Conception Center (An ISO 9002 Organization), Karnataka, India, Bangalore Assisted Conception Centre
2. Milann—The Fertility Center, Bengaluru, Karnataka, India, Genia Fertility Center, Bengaluru, Karnataka, India, Genea Fertility Center, Bengaluru, Karnataka, India, Milann JP Nagar, Bengaluru, Karnataka, India, Milann - The Fertility Center, Bengaluru, Karnataka, India, Milann—The Fertility Center (A Unit of BACC Healthcare Pvt Ltd), Bengaluru, Karnataka, India
3. BACC Healthcare Pvt Ltd, Bengaluru, Karnataka, India, Milann—The Fertility Center, Bengaluru, Karnataka, India, Milann—The Fertility Center, Bengaluru, Karnataka, Milann Fertility, Bengaluru, Karnataka, India
4. Milann—The Fertility Center (A Unit of BACC Healthcare Pvt Ltd), Mangalore, Karnataka, India, Ovum Fertility and Women's Health Center, Bengaluru, Karnataka, India, Craft Fertility Center, Gunasheela IVF Center, Hamad Medical Corporation KMC, YMC, Milann, Qatar, India, Sweden
5. Milann—The Fertility Center (A Unit of BACC Healthcare Pvt Ltd, Bengaluru, Karnataka, India, Milann—The Fertility Center, Bengaluru, Karnataka, India
6. Milann—The Fertility Center, Bengaluru, Karnataka, India, Milann Fertility Centre, Bengaluru, Karnataka, India, Milann Fertility Center, Bengaluru, Karnataka, India
7. Milann—The Fertility Center, Bengaluru, Karnataka, India
Chapter keywords
Physiological intracytoplasmic sperm injection, PICSI, intracytoplasmic morphologically selected sperm injection, poloscopy, time-lapse imaging, TLI, laser-assisted hatching, magnetic-activated cell sorting

Abstract

Physiological intracytoplasmic sperm injection (PICSI), intracytoplasmic morphologically selected sperm injection, poloscopy, time-lapse imaging (TLI), laser-assisted hatching, magnetic-activated cell sorting (MACS) of human spermatozoa are some advance techniques which are covered in this chapter. PICSI represents a physiologic alternative for slowing sperm motility before intracytoplasmic sperm injection (ICSI), avoiding any potential damaging effect of synthetic polyvinylpyrrolidone (PVP). ICSI is usually performed at an optical magnification of 400×, since this magnification allows identification of only major morphological defects of the sperm; minor morphological defects of the sperms, which seem to be related to ICSI outcome, can be missed. Polarized light microscopy (poloscopy) helps to visualize the meiotic spindle noninvasively and in a dynamic fashion. TLI is a powerful tool for the study of early embryo development. It offers many advantages over traditional time-point microscopy. MACS was introduced as a technique that separates apoptotic spermatozoa from nonapoptotic ones.

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