Amebiasis caused due to Entamoeba histolytica (E. histolytica). They usually spread by fecal contamination of food and water. Antiamebic drugs are classified into tissue amebicides and luminal amebicides. Tissue amebicides attain high tissue concentration following oral/parenteral administration. Luminal amebicides poorly absorbed after oral administration. Attain high concentration in bowel and acts on mucosal cysts and trophozoites. Metronidazole is highly effective against most anaerobic bacteria, protein such as E. histolytica, Giardia lamblia, Trichomonas vaginalis, and Balantidium coli. Treatment of amebiasis is also covered in this chapter.