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Chapter-23 Estimation of Urea

BOOK TITLE: Simplified Practical Manual of Biochemistry

Author
1. Gogoi Javin Bishnu
ISBN
9789352702619
DOI
10.5005/jp/books/14191_24
Edition
2/e
Publishing Year
2018
Pages
5
Author Affiliations
1. VCSG Government Institute of Medical Science and Research, Srinagar, Garhwal, Uttarakhand, India, Veer Chandra Singh Garhwali (VCSG), Government Institute of Medical Science and Research, Srinagar, Pauri Garhwal, Uttarakhand, India
Chapter keywords
Urea, nonprotein nitrogen, NPN, glomerular filteration rate, GFR, Nessler’s method, Berthelot reaction, glutamate dehydrogenase method

Abstract

Urea is the main end product of protein catabolism. It is formed in the liver and is excreted through urine. Urea represents about 45–50% of the nonprotein nitrogen (NPN) of blood and 80–90% of the total urinary nitrogen excretion. The urea concentration in the glomerular filtrate is same as that in plasma. Tubular reabsorption of urea varies inversely with the rate of urine flow and hence is not a useful measure of glomerular filtration rate (GFR). Various methods in use for estimation of urea are Nessler’s method, Berthelot reaction, and urease or glutamate dehydrogenase method.

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