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Chapter-17 Electrophoresis

BOOK TITLE: Simplified Practical Manual of Biochemistry

Author
1. Gogoi Javin Bishnu
ISBN
9789352702619
DOI
10.5005/jp/books/14191_18
Edition
2/e
Publishing Year
2018
Pages
5
Author Affiliations
1. VCSG Government Institute of Medical Science and Research, Srinagar, Garhwal, Uttarakhand, India, Veer Chandra Singh Garhwali (VCSG), Government Institute of Medical Science and Research, Srinagar, Pauri Garhwal, Uttarakhand, India
Chapter keywords
Electrophoresis, biological fluid, biochemistry, nephritic syndrome, multiple myeloma, paper electrophoresis, immune electrophoresis, capillary electrophoresis

Abstract

The objective of this chapter is to introduce the electrophoresis. Electrophoresis is the method of separation of macromolecules of biological fluids (e.g. serum, urine, CSF, etc.) by the movement of charged particles through an electrolyte when subjected to an electric field. As such, it is one of the most widely-used techniques in biochemistry and molecular biology. Principles of electrophoresis, types of electrophoresis, and uses of electrophoresis are also discussed in this chapter. It is widely used for separation of proteins in biological fluids in various conditions like identification of different types of Hb in hemoglobinopathies, in chronic infections, cirrhosis of liver, nephritic syndrome, and multiple myeloma (Bence Jone’s protein).

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