In general, five spots are displayed for identification in the MBBS 2nd professional Microbiology practical examination. The spots which may be generally selected in the examination are tabulated in this chapter. After reading this chapter, the readers are able to identify common spots during practical microbiology examination which include culture media; culture media with growth; biochemical reactions; specimens of parasites; specimens of mycology; specimens of virology; microscopic slides; glasswares; specimens of immunology; specimens of disposable items; equipments/instruments; medical entomology; and miscellaneous spots.