Gram stain, Ziehl-Neelsen stain, and Albert’s stain are the different types of staining methods which are discussed throughout this chapter. Gram stain is done for bacterial culture (solid material, such as cultures on agar) which is provided to the student along with liquid culture of the same material in a test tube which is used for hanging drop preparation to observe the motility of bacteria. A fixed smear is provided for Ziehl-Neelsen stain. If the smear is unfixed, it is fixed by passing the dried slide, film downwards, three times slowly through the flame, or by heating through the glass slide in the top of the Bunsen flame for a few seconds so that the slide becomes hot. Albert’s stain is used for staining of volutin-containing organisms. In Albert’s stain, metachromatic (volutin) granules stain bluish black and bacterial protoplasm green.