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Chapter-09 Staining

BOOK TITLE: Techniques in Histopathology and Cytopathology

Author
1. Vishwakarma Sadhana
ISBN
9789352701094
DOI
10.5005/jp/books/14170_10
Edition
1/e
Publishing Year
2017
Pages
29
Author Affiliations
1. Technocrats Institute of Technology, Bhopal, Madhya Pradesh, India
Chapter keywords
Staining, dyes, staining process, staining procedure, hematoxylin stains, counter stains, standard hematoxylin method, eosin staining method

Abstract

Staining is a technique used in microscopy to improve contrast in the microscopic image. Changes in tissue contrast can be brought about by microscopical methods, such as phase contrast or the use of polarized light. These make parts of the tissue gray or black. More often, histological staining methods depend on the production of colors in the tissues by dye—staining, which modifies the wavelength of the light that has been passed through the stained tissue, thereby imparting color into the tissue and its cells. Successful staining methods are both specific and sensitive. This chapter covers the principle of staining, types of dyes and stains, classification of dyes or stains, types of staining processes, staining procedures, hematoxylin stains, counter stains, standard hematoxylin and eosin staining method for paraffin sections, special staining methods, staining of frozen sections, precautions taken during staining, and causes of poor staining. In this chapter, questions for exercise and objective type questions are also provided.

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