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Chapter-07 Microtomy and Section Cutting

BOOK TITLE: Techniques in Histopathology and Cytopathology

Author
1. Vishwakarma Sadhana
ISBN
9789352701094
DOI
10.5005/jp/books/14170_8
Edition
1/e
Publishing Year
2017
Pages
16
Author Affiliations
1. Technocrats Institute of Technology, Bhopal, Madhya Pradesh, India
Chapter keywords
Microtomy, microscopic examination, microtome, microtome knives, section cutting, knife adjustment, honing, knife sharpening, vibrating microtome

Abstract

The embedded tissues are then subjected to section cutting. This involves the technique of microtomy which yields thin sections of the tissue for microscopic examination of the internal structure. Sections are prepared on an instrument known as a microtome of which various types are available. Common features of all the microtomes are that they are having firm support for knife and tissue block and a feed mechanism designed to advance the specimen a predetermined number of micrometers on each cutting stroke. This chapter covers the microtomes, microtome knives, section cutting, attaching the sections to slides, and problems and remedies encountered during mounting of the sections. In this chapter, questions for exercise and objective type questions are also provided.

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