This chapter provides detail description of ophthalmic histopathology in medical laboratory technique. Chapter has 29 well illustrated diagrams and pictures. Specimens entry, specimen fixation, grossing technique, decalcification, tissue processing, embedding, frozen section, staining techniques, immunohistochemistry, cytology study, storing process, special stains are described in detail with the help of excellent illustrations. 1 to 2 references are given at the end of each topic for further reading. 2 tables are included to highlight standard protocols of different techniques.