This chapter provides detail description of establishing an ART unit in low source setting. Chapter has 1 well illustrated diagram. Hysterosalpingography, cost of ART, in vitro maturation of oocytes, controlled ovarian stimulation; follicular tracking, intravaginal culture and single embryo transfer are described in detail. One flow chart is added to highlight infertility work up map. IVF laboratory set up map is included for better understanding. 48 references are included at the end of the chapter to facilitate further reading.