This chapter provides detail description of in vitro maturation. Chapter has 1 well illustrated diagram and picture. In vitro maturation, oocytes from antral follicles, cytoplasmic maturity in oocytes, insemination after IVM, oocyte ageing, embryonic development, IVM media, IVM for fertility preservation are described in detail. 1table is included to highlight list of recent culture conditions. 14 references are included at the end of the chapter to facilitate further reading.