The overall outcomes after liver transplantation have improved significantly, but early graft failure still remains a serious concern because it is associated with higher morbidity and mortality. Cold ischemia and subsequent reperfusion are crucial factors for graft outcome in organ transplantation. Once the graft is harvested, graft PV is flushed with preservation solution and preserved in cold preservation solution. The introduction of UW in liver transplantation improved graft and patient survival. Components like impermeants, an effective buffering system, and the provision of substrates to regenerate high-energy phosphates may have contributed to this result. HTK was originally developed for heart preservation and was subsequently introduced in the preservation of human liver grafts.