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Chapter-16 Estimation of blood glucose by O-toluidine method

BOOK TITLE: Laboratory Manual for Practical Biochemistry

Author

1. MK Ganesh
2. A Hemavathi
3. YM Shivaraja Shankara

ISBN

9788184482591

DOI

10.5005/jp/books/10435_17

Edition

1/e

Publishing Year

2008

Pages

Author Affiliations

1. KVG Dental College, Sullia, Karnataka, KVG Dental College, Sullia, Karnataka, India
2. KVG Medical College, Sullia, Karnataka
3. KVG Medical College, Sullia, Karnataka, KVG Medical College, Sullia, Karnataka, India

Chapter keywords

Abstract

Abstract

Proteins in blood are precipitated with trichloroacetic acid. The protein free filtrate containing glucose reacts with O-toluidine in glacial acetic acid in the presence of heat to form green colored N-glycosylamine. The intensity of green color is proportional to the amount of glucose present and the color intensity is measured by using a red filter (625 nm).

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