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Chapter-15 Estimation of blood sugar by Folin-Wu method

BOOK TITLE: Laboratory Manual for Practical Biochemistry

Author
1. MK Ganesh
2. A Hemavathi
3. YM Shivaraja Shankara
ISBN
9788184482591
DOI
10.5005/jp/books/10435_16
Edition
1/e
Publishing Year
2008
Pages
5
Author Affiliations
1. KVG Dental College, Sullia, Karnataka, KVG Dental College, Sullia, Karnataka, India
2. KVG Medical College, Sullia, Karnataka
3. KVG Medical College, Sullia, Karnataka, KVG Medical College, Sullia, Karnataka, India
Chapter keywords

Abstract

Estimation of blood sugar virtually means estimation of blood glucose level. In this method, proteins in blood are precipitated with tungstic acid. The protein free filtrate containing glucose reduces cupric ions (Cu2+) to cuprous ions (Cu+) and forms cuprous oxide (Cu2O). Cuprous oxide then reduces phosphomolybdic acid to blue phosphomolybdous acid (Molybdenum blue). The intensity of blue color is proportional to the amount of glucose present, and the color intensity is measured by using a blue filter (490 nm).

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