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Chapter-14 Molecular Techniques in Hematology

BOOK TITLE: Laboratory Techniques in Haematology

Author
1. R Saxena
2. A Biswas
3. M Kannan
4. S Sazawal
ISBN
9788184482201
DOI
10.5005/jp/books/10438_14
Edition
1/e
Publishing Year
2008
Pages
60
Author Affiliations
1. AIIMS, New Delhi, All India Institute of Medical Sciences, New Delhi, India, Max Super Speciality Hospital, Shalimar Bagh, New Delhi, India
2. AIIMS, New Delhi
3. AIIMS, New Delhi
4. AIIMS, New Delhi
Chapter keywords

Abstract

This chapter describes basic molecular techniques of use in hematology. These include detailed protocol for DNA extraction, RNA extraction, southern blotting techniques, PCR, dot blot and reverse dot blot techniques, RT PCR, Gap PCR, PCR-RFLP and real time PCR. It also describes method of mutation screening such as SSCP, DGGE, CSGE, DHPLC.

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