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Chapter-27 Buffy Coat Preparation of Peripheral Blood and Bone Marrow

BOOK TITLE: Laboratory Procedures in Haematology

Author
1. Mehdi SR
ISBN
9788180616570
DOI
10.5005/jp/books/10437_27
Edition
1/e
Publishing Year
2006
Pages
4
Author Affiliations
1. JN Medical College, AMU, Aligarh, India, Era’s Lucknow Medical College, Lucknow, Uttar Pradesh, India
Chapter keywords

Abstract

The buffy coat preparation of blood is commonly employed in demonstration of LE cell. Buffy coat is invariably required in study of abnormal cells in all leukopenic patients. Centrifugation and separation of the top creamy layer of cells yields buffy coat which is rich in leukocytes. Capillary tube, an ordinary test tube or a Wintrobe tube can be used for centrifugation and collection of buffy coat.

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