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47 Result(s) for 'SubCategory='Biotechnology''
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Biotechnology—Foundation Course by N Rao Ananth (2007) Edition : 1/e
DOI : 10.5005/jp/books/10104   ISBN:9788180619984

The book is intended to serve as a foundation for beginners in the field of biotechnology. The book covers a wide range of topics such as Classical Genetics, Eugenics, Biochemistry, Biochemical Techniques, General Microbiology, Industrial Microbiology and Immunology, etc. It is indeed rare for such a comprehensive range under one book. The book has drawn inf... [More]

Biotechnology—Foundation Course
Chapter-01 Cell Culture by Ramadass P, Aruni A Wilson (2007) Edition : 1/e

This chapter discusses the use of cell culture technology in diagnostic virology. The do’s and don’ts in this technology, materials used, transport medium, growth medium, basic constituents of media, and tissue culture media, are discussed in detail. The regulatory requirements in Europe stress the importance of justifying the use of material of bovine, capr... [More]

Chapter-01 Introduction by Rao Ananth N (2007) Edition : 1/e

Biochemistry, Carbohydrates, Electrostatic bonds, Hydrogen bonds, Lipids, Living organisms, Lonic bonds, Nucleic acids, Organic chemistry, Proteins, Sodium chloride crystal, Study of biology, Van der Waals forces

Chapter-01 Preparation of Tissues with Implants for Microscopy by Sabareeswaran A, Sebastian Joseph, Mohanty Mira, Baby Sulekha (2013) Edition : 1/e

This chapter takes the reader through a brief description of all the steps by which a tissue with or without an implant should be processed to obtain a thin section for viewing under the microscope. A tissue section provides important information of the morphological changes in the cells and tissues in response to the implanted material. The section must pro... [More]

Chapter-02 Implants in Soft Tissue by Sabareeswaran A, Sebastian Joseph, Mohanty Mira, Baby Sulekha (2013) Edition : 1/e

This chapter provides visual information of changes which take place at the tissue-material interface following implantation of a material in soft tissue such as muscle and subcutaneous tissue. The sequences of events which take place beginning with tissue necrosis, progressing to inflammation and ending with repair can be understood with explanatory caption... [More]

Chapter-02 Monoclonal Antibody Production by Ramadass P, Aruni A Wilson (2007) Edition : 1/e

This chapter discusses the role of monoclonal antibodies for research and medical procedures. Topics discussed include generation of monoclonal antibodies, cell fusion, separation of proteins by SDS-page, immunoblotting using a chemiluminescence detection system, ascetic fluid production, and preparation of peritoneal macrophages. Myelomas can be induced in ... [More]

Chapter-03 DNA and RNA Isolation and Electrophoresis by Ramadass P, Aruni A Wilson (2007) Edition : 1/e

This chapter discusses use of various DNA and RNA isolation and electrophoresis techniques in biotechnology. Various protocols studied include mini preparation of plasmid, phagemid or RF of M13 DNA, large-scale preparation of plasmid, phagemid or RF of M13 DNA, purification of dsDNA fragments from LMP agarose, purification of dsDNA fragment by electroelution... [More]

Chapter-03 Enzymes by Rao Ananth N (2007) Edition : 1/e

Enzymes, Classification of enzymes, Compartmentalization, Covalent activation of zymogens, CPK, Effect of pH, Effect of temperature, Enzyme activity units, Enzyme induction, Repression, Enzyme inhibition, Enzyme regulation, Isozymes, Kinetics of bisubstrate reactions, Kinetics of enzyme, Michaelis-menton equation, Multienzyme systems, Physical chemistry, Sin... [More]

Chapter-03 Implants in Bone by Sabareeswaran A, Sebastian Joseph, Mohanty Mira, Baby Sulekha (2013) Edition : 1/e

This chapter describes the importance of examining the tissue-material interface around materials implanted in bone. The photomicrographs bring out cellular details in tissue at the interface, helping the reader to differentiate between bone and fibrous tissue. The advantage of resin embedded sections which contain the bone with implant, thus preserving the ... [More]